Search results for "Aristolochic acid"

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Implication of three isoforms of PLA2in human T-cell proliferation

2002

We observed that human (Jurkat) T-cells constitutively expressed the mRNA, encoding for the four isoforms of phospholipase A(2) (PLA(2)), i.e. two secretory (type IB and type V), and two cytosolic (type IV, Ca(2+)-dependent and type VI, Ca(2+)-independent). In order to assess whether these PLA(2) isoforms are active, we labeled Jurkat T-cells with [(3)H]arachidonic acid ([(3)H]AA) and determined its release into the extracellular medium in the presence of phorbol 12-myristate 13-acetate (PMA) and ionomycin. The three PLA(2) isoforms seem functional as aristolochic acid and bromoenol lactone (BEL), the respective inhibitors of type IB/type V and type VI PLA(2)s, significantly inhibited the r…

BiophysicsAristolochic acidArachidonic AcidsPhospholipaseTritiumBiochemistryJurkat cellsGene Expression Regulation EnzymologicPhospholipases AJurkat Cellschemistry.chemical_compoundPhospholipase A2Structural BiologyGeneticsHumansPhospholipaseRNA MessengerEnzyme InhibitorsMolecular BiologyArachidonyl trifluoromethyl ketoneArachidonic AcidbiologyIonomycinCell BiologyJurkat T-cellIsoenzymesGene Expression RegulationchemistryBiochemistryIonomycinPhorbolbiology.proteinInterleukin-2Tetradecanoylphorbol AcetateCalciumlipids (amino acids peptides and proteins)Arachidonic acidCell DivisionFEBS Letters
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